ABSTRACT
Free radicals are generated by both enzymatic and non-enzymatic reactions. The main source of free radicals in enzymatic reactions include those involved in phagocytosis, respiratory chain, in prostaglandin synthesis, and in cytochrome p450 system. Current investigation was to examine invitro antioxidant potential of Cassia absus (Linn). MethodsThe aerial parts of Cassia absus (Linn) were powdered and the dry powder was subjected to extraction with various solvents (PE, EA and methanol) through Soxhlet extractor. The aerial parts of different concentrates (pet. ether, ethyl acetate and methanol) of Cassia absus was evaluated for its in-vitro antioxidant potential by hydroxyl radical, FRAP activity taking ascorbate used as standard for the both methods and total flavonoids content was estimated as equivalent to rutin. ResultsThe methanolic concentrates of Cassia absus & standard exhibited antioxidant potential possessing IC50 196 µg/mL & 65 µg/mL (hydroxyl radical) 216 µg/mL & 50 µg/mL (FRAP activity) respectively. Methanolic concentrates of Cassia absus were more efficient in hydroxyl radical, FRAP activity compared EA & PE concentrates. The methanolic and EA concentrates of Cassia absus showed the total flavonoids content (10.22 ± 0.40, 4.03 ± 0.47 respectively). The difference in scavenging potential of the extracts can be due to variation in the percentage of bioactive compound flavonoids present in methanolic extracts. Invitro antioxidant studies show that methanolic concentrates of Cassia absus have better antioxidant activity due to the presence of total flavonoids content. ConclusionsThis result indicates that methanolic concentrates of aerial parts of Cassia absus could serve as natural antioxidant, which may be useful in preventing free radical induced diseases.
ABSTRACT
The study was carried out to determine the in vivo antioxidant and lipid peroxidation effect of methanolic extract of tuberous root of Ipomoea digitata (Linn). High fat diet rats showed significantly (P<0.001) reduced the levels of tissues enzymatic antioxidant and enhanced the level of non enzymatic antioxidant (Glutathione). The level of TBARS are elevated in HFD rats (group II) when compared with control group. High fat diet induces the oxidative stress in cell by producing reactive oxygen species. Administration of methanolic extract of Ipomoea digitata in high fat diet rats were showed increased the levels of antioxidant Enzymes such as Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GPx), Glutathione reductase (GR) and reduced the level of non enzymatic antioxidant Glutathione (GSH) when compared with HFD rats (Group II). The methanolic extract Ipomoea digitata in high fat diet rats were found lowered the concentration of TBARS when compared with HFD rats. Based on the results, we concluded that the methanolic extract of Ipomoea digitata is a significant source of natural antioxidant, which might be helpful in preventing the progress of various oxidative stresses.
ABSTRACT
High fat diet fed rats showed significant increased levels of plasma and tissue total cholesterol, triglycerides, free fatty acids, phospholipids, plasma LDL cholesterol and decreased level of plasma HDL cholesterol. Methanolic extract of D. biflorus administration to high fat diet fed rats showed near to normal levels of the above lipids in plasma and tissues. Higher dose of the extract (400 mg/kg body weight) showed comparable results with standard drug atorvastatin. It is concluded that the methanolic extract of D. biflorus possesses hypolipidemic activity in high fat diet fed rats.